packages feed

sequence-formats (empty) → 1.1.4.1

raw patch · 10 files changed

+1396/−0 lines, 10 filesdep +MissingHdep +attoparsecdep +base

Dependencies added: MissingH, attoparsec, base, bytestring, containers, data-memocombinators, errors, exceptions, foldl, hslogger, lens-family, pipes, pipes-attoparsec, pipes-bytestring, pipes-safe, pipes-text, split, text, transformers, turtle, vector

Files

+ Changelog.md view
@@ -0,0 +1,1 @@+V 1.1.4.1: First entry in the Changelog. Added Haddock documentation to all modules and prepare for releasing on Hackage.
+ LICENSE view
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+ README.md view
@@ -0,0 +1,3 @@+Sequence-Formats is a Haskell package to provide file parsers and writers for some commonly, and less commonly used file formats in Bioinformatics, speficially population genetics.++The library makes heavy use of the [pipes library](http://hackage.haskell.org/package/pipes) to provide Producers and Consumers for reading and writing files.
+ sequence-formats.cabal view
@@ -0,0 +1,48 @@+cabal-version: >=1.10+name: sequence-formats+version: 1.1.4.1+license: GPL-3+license-file: LICENSE+maintainer: stephan.schiffels@mac.com+author: Stephan Schiffels+synopsis: A package with basic parsing utilities for several Bioinformatic data formats.+description:+    Contains utilities to parse and write Eigenstrat, Fasta, FreqSum, VCF and other file formats used in population genetics analyses.+category: Bioinformatics+build-type: Simple+extra-source-files:+    README.md+    Changelog.md++library+    exposed-modules:+        SequenceFormats.RareAlleleHistogram+        SequenceFormats.FreqSum+        SequenceFormats.Fasta+        SequenceFormats.VCF+        SequenceFormats.Eigenstrat+        SequenceFormats.Utils+    hs-source-dirs: src+    default-language: Haskell2010+    build-depends:+        base >=4.7 && <5,+        containers >=0.5.10.2 && <0.6,+        errors >=2.2.2 && <2.3,+        text >=1.2.2.2 && <1.3,+        attoparsec >=0.13.2.2 && <0.14,+        pipes >=4.3.7 && <4.4,+        pipes-text >=0.0.2.5 && <0.1,+        transformers >=0.5.2.0 && <0.6,+        turtle >=1.4.5 && <1.5,+        bytestring >=0.10.8.2 && <0.11,+        lens-family >=1.2.2 && <1.3,+        pipes-bytestring >=2.1.6 && <2.2,+        foldl >=1.3.7 && <1.4,+        exceptions >=0.8.3 && <0.9,+        pipes-safe >=2.2.6 && <2.3,+        pipes-attoparsec >=0.5.1.5 && <0.6,+        vector >=0.12.0.1 && <0.13,+        data-memocombinators >=0.5.1 && <0.6,+        split >=0.2.3.3 && <0.3,+        MissingH >=1.4.0.1 && <1.5,+        hslogger >=1.2.10 && <1.3
+ src/SequenceFormats/Eigenstrat.hs view
@@ -0,0 +1,168 @@+{-# LANGUAGE OverloadedStrings #-}++{-|Module to read and parse Eigenstrat-formatted genotype data. The Eigenstrat format is defined at <https://github.com/argriffing/eigensoft/blob/master/CONVERTF/README>.++-}++module SequenceFormats.Eigenstrat (EigenstratSnpEntry(..), EigenstratIndEntry(..), +    readEigenstratInd, GenoEntry(..), GenoLine, Sex(..), +    readEigenstratSnpStdIn, readEigenstratSnpFile, readEigenstrat, writeEigenstrat) where++import SequenceFormats.Utils (consumeProducer, FormatException(..), Chrom(..))++import Control.Applicative ((<|>))+import Control.Monad (void, forM_)+import Control.Monad.Catch (MonadThrow, throwM)+import Control.Monad.IO.Class (MonadIO, liftIO)+import qualified Data.Attoparsec.Text as A+import Data.Char (isSpace)+import Data.Vector (Vector, fromList, toList)+import qualified Data.Text as T+import qualified Data.Text.IO as T+import Pipes (Producer, Pipe, (>->), for, cat, yield, Consumer)+import Pipes.Safe (MonadSafe)+import qualified Pipes.Prelude as P+import qualified Pipes.Text.IO as PT+import System.IO (withFile, IOMode(..))+import Turtle (format, w, d, (%), s)++-- |A datatype to represent a single genomic SNP. The constructor arguments are:+-- Chromosome, Position, Reference Allele, Alternative Allele.+data EigenstratSnpEntry = EigenstratSnpEntry Chrom Int Char Char deriving (Eq, Show)++-- |A datatype to represent a single individual. The constructor arguments are:+-- Name, Sex and Population Name+data EigenstratIndEntry = EigenstratIndEntry T.Text Sex T.Text deriving (Show)++-- |A datatype to represent Sex in an Eigenstrat Individual file+data Sex = Male | Female | Unknown deriving (Show)++-- |A datatype to represent the genotype of an individual at a SNP.+data GenoEntry = HomRef | Het | HomAlt | Missing deriving (Eq, Show)++-- |Vector of the genotypes of all individuals at a single SNP.+type GenoLine = Vector GenoEntry++eigenstratSnpParser :: A.Parser EigenstratSnpEntry+eigenstratSnpParser = do+    A.skipMany A.space+    void word+    A.skipMany1 A.space+    chrom <- word+    A.skipMany1 A.space+    void word+    A.skipMany1 A.space+    pos <- A.decimal+    A.skipMany1 A.space+    ref <- A.satisfy (A.inClass "ACTGN")+    A.skipMany1 A.space+    alt <- A.satisfy (A.inClass "ACTGX")+    void A.endOfLine+    return $ EigenstratSnpEntry (Chrom chrom) pos ref alt++word :: A.Parser T.Text+word = A.takeTill isSpace++eigenstratIndParser :: A.Parser EigenstratIndEntry+eigenstratIndParser = do+    A.skipMany A.space+    name <- word+    A.skipMany1 A.space+    sex <- parseSex+    A.skipMany1 A.space+    popName <- word+    void A.endOfLine+    return $ EigenstratIndEntry name sex popName++parseSex :: A.Parser Sex+parseSex = parseMale <|> parseFemale <|> parseUnknown+  where+    parseMale = A.char 'M' >> return Male+    parseFemale = A.char 'F' >> return Female+    parseUnknown = A.char 'U' >> return Unknown++-- |Function to read an Eigenstrat individual file. Returns the Eigenstrat Individual Entries as list.+readEigenstratInd :: (MonadIO m) => FilePath -> m [EigenstratIndEntry]+readEigenstratInd fn = do+    liftIO . withFile fn ReadMode $ \handle -> do+        P.toListM $ consumeProducer eigenstratIndParser (PT.fromHandle handle)++eigenstratGenoParser :: A.Parser GenoLine+eigenstratGenoParser = do+    line <- A.takeWhile1 isValidNum+    void A.endOfLine+    return . fromList $ do+        l <- T.unpack line+        case l of+            '0' -> return HomAlt+            '1' -> return Het+            '2' -> return HomRef+            '9' -> return Missing+            _ -> error "this should never happen"+  where+    isValidNum c = c == '0' || c == '1' || c == '2' || c == '9'++-- |Function to read a Snp File from StdIn. Returns a Pipes-Producer over the EigenstratSnpEntries.+readEigenstratSnpStdIn :: (MonadThrow m, MonadIO m) => Producer EigenstratSnpEntry m ()+readEigenstratSnpStdIn = consumeProducer eigenstratSnpParser PT.stdin++-- |Function to read a Snp File from a file. Returns a Pipes-Producer over the EigenstratSnpEntries.+readEigenstratSnpFile :: (MonadSafe m) => FilePath -> Producer EigenstratSnpEntry m ()+readEigenstratSnpFile = consumeProducer eigenstratSnpParser . PT.readFile++-- |Function to read a full Eigenstrat database from files. Returns a pair of the Eigenstrat Individual Entries, and a joint Producer over the snp entries and the genotypes.+readEigenstrat :: (MonadSafe m) => FilePath -- ^The Genotype file+               -> FilePath -- ^The Snp File+               -> FilePath -- ^The Ind file+               -> m ([EigenstratIndEntry], Producer (EigenstratSnpEntry, GenoLine) m ()) -- The return pair of individual entries and a joint Snp/Geno Producer.+readEigenstrat genoFile snpFile indFile = do+    indEntries <- readEigenstratInd indFile+    let snpProd = readEigenstratSnpFile snpFile+        genoProd = consumeProducer eigenstratGenoParser (PT.readFile genoFile) >-> +            validateEigenstratEntries (length indEntries)+    return (indEntries, P.zip snpProd genoProd)++validateEigenstratEntries :: (MonadThrow m) => Int -> Pipe GenoLine GenoLine m ()+validateEigenstratEntries nr = for cat $ \line -> do+    if length line /= nr+    then do+        let msg = format ("inconsistent nr of genotypes ("%d%", but should be "%d%") in \+                \genotype line "%w) (length line) nr line+        throwM $ FormatException msg+    else+        yield line++-- |Function to write an Eigenstrat Database. Returns a consumer expecting joint Snp- and Genotype lines.+writeEigenstrat :: (MonadSafe m) => FilePath -- ^The Genotype file+                -> FilePath -- ^The Snp File+                -> FilePath -- ^The Ind file+                -> [EigenstratIndEntry] -- ^The list of individual entries+                -> Consumer (EigenstratSnpEntry, GenoLine) m () -- ^A consumer to read joint Snp/Genotype entries.+writeEigenstrat genoFile snpFile indFile indEntries = do+    liftIO $ writeEigenstratIndFile indFile indEntries+    let snpOutTextConsumer = PT.writeFile snpFile+        genoOutTextConsumer = PT.writeFile genoFile+        toTextPipe = P.map (\(EigenstratSnpEntry chrom pos ref alt, genoLine) ->+            let n = format (s%"_"%d) (unChrom chrom) pos+                snpLine = format (s%"\t"%s%"\t0\t"%d%"\t"%s%"\t"%s%"\n") n (unChrom chrom) pos+                    (T.singleton ref) (T.singleton alt)+                genoLineStr = T.concat . map (format d . toEigenStratNum) . toList $ genoLine+            in  (snpLine, format (s%"\n") genoLineStr))+    toTextPipe >-> P.tee (P.map fst >-> snpOutTextConsumer) >-> P.map snd >-> genoOutTextConsumer+  where+    toEigenStratNum c = case c of+        HomRef -> 2 :: Int+        Het -> 1+        HomAlt -> 0+        Missing -> 9++writeEigenstratIndFile :: (MonadIO m) => FilePath -> [EigenstratIndEntry] -> m ()+writeEigenstratIndFile f indEntries = do+    liftIO . withFile f WriteMode $ \h -> do+        forM_ indEntries $ \(EigenstratIndEntry name sex popName) -> do+            liftIO . T.hPutStrLn h $ format (s%"\t"%s%"\t"%s) name (sexToStr sex) popName+  where+    sexToStr sex = case sex of+        Male -> "M"+        Female -> "F"+        Unknown -> "U"
+ src/SequenceFormats/Fasta.hs view
@@ -0,0 +1,67 @@+{-# LANGUAGE OverloadedStrings #-}++{-| Module to read and parse through a Fasta file. The Fasta format is defined here:+<https://en.wikipedia.org/wiki/FASTA_format>+-}+module SequenceFormats.Fasta (readNextFastaEntry, loadFastaChrom) where++import SequenceFormats.Utils (Chrom(..))++import Control.Exception.Base (throwIO, AssertionFailed(..))+import Control.Monad (void)+import Control.Monad.IO.Class (liftIO, MonadIO)+import Control.Monad.Trans.State.Strict (runStateT)+import qualified Data.Attoparsec.ByteString.Char8 as A+import qualified Data.ByteString.Char8 as B+import Data.Char (isAlphaNum)+import qualified Data.Text as T+import Lens.Family2 (view)+import Pipes (Producer, next, (>->), runEffect)+import Pipes.Attoparsec (parse)+import qualified Pipes.ByteString as P+import Pipes.Prelude (drain)+import System.IO (Handle)+import Turtle.Format (format, (%), s)+import Turtle.Prelude (err)+import Turtle.Line (unsafeTextToLine)++-- |A function to select out a specific chromosome from a Fasta File. Expects a file handle to the +-- file and a chromosome. Note that by Chromosome I simply denote a fasta header line, as is the +-- case for example for the human reference genome. Returns a Bytestring-Producer over the single sequence followed the specified header (the chromosome).+loadFastaChrom :: Handle -> Chrom -> IO (Producer B.ByteString IO ())+loadFastaChrom refFileHandle chrom = do+    let prod = P.fromHandle refFileHandle+    go prod+  where+    go prod = do+        (chrom_, prod') <- readNextFastaEntry prod+        err . unsafeTextToLine $ format ("found chromosome "%s) (unChrom chrom_)+        if chrom_ == chrom+        then return (void prod')+        else do+            newProd <- runEffect $ prod' >-> drain+            go newProd++-- |This function takes a Bytestring-Producer over a Fasta-file, reads in the first header and then returns a produer over its sequence. The return of that producer is the Bytestring-Producer of the rest of the fasta file.+readNextFastaEntry :: (MonadIO m) => Producer B.ByteString m () ->+                      m (Chrom, Producer B.ByteString m (Producer B.ByteString m ()))+readNextFastaEntry prod = do+    (res, rest) <- runStateT (parse fastaHeaderLineParser) prod+    header <- case res of+        Nothing -> liftIO . throwIO $ AssertionFailed "Could not find chromosome. Fasta file exhausted."+        Just (Left e_) -> do+            Right (chunk, _) <- next rest+            let msg = show e_ ++ B.unpack chunk+            liftIO . throwIO $ AssertionFailed ("Fasta header parsing error: " ++ msg)+        Just (Right h) -> return h+    return (header, view (P.break (==62)) rest >-> P.filter (\c -> c /= 10 && c /= 13))+-- '>' == 62, '\n' == 10, \r == 13++fastaHeaderLineParser :: A.Parser Chrom+fastaHeaderLineParser = do+    _ <- A.char '>'+    chrom <- A.takeWhile isAlphaNum+    A.skipSpace+    A.skipWhile (\c -> c /= '\n' && c /= '\r')+    A.endOfLine+    return . Chrom . T.pack . B.unpack $ chrom
+ src/SequenceFormats/FreqSum.hs view
@@ -0,0 +1,109 @@+{-# LANGUAGE OverloadedStrings #-}++{-| Module to parse and write freqSum files. The freqsum format is defined here:+<https://rarecoal-docs.readthedocs.io/en/latest/rarecoal-tools.html#vcf2freqsum>+-}++module SequenceFormats.FreqSum (readFreqSumStdIn, readFreqSumFile, FreqSumEntry(..),  +    FreqSumHeader(..), printFreqSumStdOut, printFreqSumFile, freqSumEntryToText) where++import SequenceFormats.Utils (consumeProducer, Chrom(..))++import Control.Applicative ((<|>))+import Control.Monad.Catch (MonadThrow, throwM)+import Control.Monad.IO.Class (MonadIO, liftIO)+import Control.Monad.Trans.State.Strict (runStateT)+import qualified Data.Attoparsec.Text as A+import Data.Char (isAlphaNum, isSpace)+import Data.Text (Text, intercalate, singleton)+import Data.Text.IO (putStr, hPutStr)+import Pipes (Producer, (>->), Consumer)+import Pipes.Attoparsec (parse, ParsingError(..))+import qualified Pipes.Prelude as P+import Pipes.Safe (MonadSafe)+import Pipes.Safe.Prelude (withFile)+import qualified Pipes.Text.IO as PT+import Prelude hiding (putStr)+import System.IO (IOMode(..))+import Turtle (format, s, d, (%))++-- |A Datatype representing the Header+data FreqSumHeader = FreqSumHeader {+    fshNames :: [Text], -- ^A list of individual or group names+    fshCounts :: [Int] -- ^A list of haplotype counts per individual/group.+} deriving (Eq)++freqSumHeaderToText :: FreqSumHeader -> Text+freqSumHeaderToText (FreqSumHeader names nCounts) =+    format ("#CHROM\tPOS\tREF\tALT\t"%s%"\n") (intercalate "\t" tuples)+  where+    tuples = zipWith (\n c -> format (s%"("%d%")") n c) names nCounts++-- |A Datatype to denote a single freqSum line+data FreqSumEntry = FreqSumEntry {+    fsChrom  :: Chrom, -- ^The chromosome of the site+    fsPos    :: Int, -- ^The position of the site+    fsRef    :: Char, -- ^The reference allele+    fsAlt    :: Char, -- ^The alternative allele+    fsCounts :: [Maybe Int] -- ^A list of allele counts in each group. Nothing denotes missing data.+}++-- |This function converts a single freqSum entry to a printable freqSum line.+freqSumEntryToText :: FreqSumEntry -> Text+freqSumEntryToText (FreqSumEntry chrom pos ref alt maybeCounts) =+    format (s%"\t"%d%"\t"%s%"\t"%s%"\t"%s%"\n") (unChrom chrom) pos (singleton ref) (singleton alt) +        countStr +  where+    countStr = intercalate "\t" . map (format d . convertToNum) $ maybeCounts +    convertToNum Nothing = -1+    convertToNum (Just a) = a++readFreqSumProd :: (MonadThrow m) =>+    Producer Text m () -> m (FreqSumHeader, Producer FreqSumEntry m ())+readFreqSumProd prod = do+    (res, rest) <- runStateT (parse parseFreqSumHeader) prod+    header <- case res of+        Nothing -> throwM $ ParsingError [] "freqSum file exhausted"+        Just (Left e) -> throwM e+        Just (Right h) -> return h+    return (header, consumeProducer parseFreqSumEntry rest)++-- |A function to read a freqsum file from StdIn. Returns a pair of a freqSum Header and a Producer over all lines.+readFreqSumStdIn :: (MonadIO m, MonadThrow m) => m (FreqSumHeader, Producer FreqSumEntry m ())+readFreqSumStdIn = readFreqSumProd PT.stdin++-- |A function to read a freqsum file from a file. Returns a pair of a freqSum Header and a Producer over all lines.+readFreqSumFile :: (MonadSafe m) => FilePath -> m (FreqSumHeader, Producer FreqSumEntry m ())+readFreqSumFile = readFreqSumProd . PT.readFile++parseFreqSumHeader :: A.Parser FreqSumHeader+parseFreqSumHeader = do+    tuples <- A.string "#CHROM\tPOS\tREF\tALT\t" >> A.sepBy' tuple A.space <* A.endOfLine+    let names = map fst tuples+        counts = map snd tuples+    return $ FreqSumHeader names counts+  where+    tuple = (,) <$> A.takeWhile (\c -> isAlphaNum c || c == '_' || c == '-') <* A.char '(' <*> A.decimal <* A.char ')'++parseFreqSumEntry :: A.Parser FreqSumEntry+parseFreqSumEntry = FreqSumEntry <$> (Chrom <$> A.takeTill isSpace) <* A.skipSpace <*> A.decimal <*+    A.skipSpace <*> base <* A.skipSpace <*> baseOrDot <* A.skipSpace <*> counts <* A.endOfLine+  where+    counts = (parseMissing <|> parseCount) `A.sepBy` A.char '\t'+    parseMissing = A.string "-1" *> pure Nothing+    parseCount = Just <$> A.decimal+    base = A.satisfy (A.inClass "ACTGN")+    baseOrDot = A.satisfy (A.inClass "ACTG.")++-- |A function to write freqSum data to StdOut. Expects the freqSum header as argument, and then returns a Consumer that accepts freqSum entries.+printFreqSumStdOut :: (MonadIO m) => FreqSumHeader -> Consumer FreqSumEntry m ()+printFreqSumStdOut fsh = do+    liftIO . putStr . freqSumHeaderToText $ fsh+    P.map freqSumEntryToText >-> PT.stdout++-- |A function that writes a freqSum file. Expects the FilePath and the freqSum header as arguments, and then returns a Consumer that accepts freqSum entries.+printFreqSumFile :: (MonadSafe m) => FilePath -> FreqSumHeader -> Consumer FreqSumEntry m ()+printFreqSumFile outFile fsh = do+    outFileH <- withFile outFile WriteMode return+    liftIO . hPutStr outFileH . freqSumHeaderToText $ fsh+    P.map freqSumEntryToText >-> PT.toHandle outFileH
+ src/SequenceFormats/RareAlleleHistogram.hs view
@@ -0,0 +1,116 @@+{-# LANGUAGE OverloadedStrings #-}++{-| A module to read and write allele sharing histograms, as defined here:+<https://rarecoal-docs.readthedocs.io/en/latest/rarecoal.html#histogram-files>+-}++module SequenceFormats.RareAlleleHistogram (RareAlleleHistogram(..), readHistogramFromHandle,+                            SitePattern, readHistogram, showHistogram, showSitePattern) where++import Control.Applicative (optional)+import Control.Error (Script, scriptIO, assertErr, throwE)+import Control.Monad.Trans.State.Strict (evalStateT)+import qualified Data.Attoparsec.Text as A+import Data.Char (isAlphaNum)+import Data.Int (Int64)+import Data.List (intercalate, sortBy)+import qualified Data.Map.Strict as Map+import qualified Data.Text as T+import Pipes.Attoparsec (parse)+import qualified Pipes.Text.IO as PT+import System.IO (Handle, openFile, IOMode(..), hClose)+import Turtle.Format ((%), w, format)++-- |A datatype to represent an Allele Sharing Histogram:+data RareAlleleHistogram = RareAlleleHistogram {+    raNames :: [T.Text], -- ^A list of branch names+    raNVec :: [Int], -- ^A list of haploid sample sizes.+    raMinAf :: Int, -- ^The minimum allele count+    raMaxAf :: Int, -- ^The maximum allele count+    raConditionOn :: [Int], -- ^A list of branch indices that were used to condition the allele +                            --sharing pattern+    raExcludePatterns :: [SitePattern], -- ^A list of patterns that are excluded.+    raTotalNrSites :: Int64, -- ^The total number of non-missing sites in the genome.+    raCounts :: Map.Map SitePattern Int64, -- ^The actual data, a dictionary from allele sharing patterns to observed numbers.+    raJackknifeEstimates :: Maybe (Map.Map SitePattern (Double, Double)) -- ^An optional dictionary that contains Jackknife estimates and standard deviations for each pattern frequency.+}++-- |A simple type synonym for the SitePattern, represented as a list of Integers that represents +-- each pattern across the branches.+type SitePattern = [Int]++-- |A simple function to convert a pattern into a String.+showSitePattern :: SitePattern -> String+showSitePattern nVec = intercalate "," . map show $ nVec++-- |Function to convert a Rare Allele Histogram to text. Returns an error if attempting to print a +-- histogram with non-standard settings. Many settings, such as minAf>1, are only meant for +-- in-memory representations, but are not compatible with the file format itself.+showHistogram :: RareAlleleHistogram -> Either T.Text T.Text+showHistogram hist = do+    assertErr "can only print histogram with minAf=1 due to format-legacy" $ raMinAf hist == 1+    assertErr "can only print histogram with no conditioning due to format-legacy" $+        null (raConditionOn hist)+    assertErr "can only print histogram with no exclude pattern due to format-legacy" $+        null (raExcludePatterns hist)+    let head0 = T.concat ["NAMES=", T.intercalate "," . raNames $ hist]+        head1 = T.concat ["N=", T.pack . intercalate "," . map show . raNVec $ hist]+        head2 = T.concat ["MAX_M=", T.pack . show . raMaxAf $ hist]+        head3 = T.concat ["TOTAL_SITES=", T.pack . show . raTotalNrSites $ hist]+        body = do+            (k, v) <- sorted+            case raJackknifeEstimates hist of+                Nothing -> [T.intercalate " " [T.pack . showSitePattern $ k, T.pack . show $ v]]+                Just jkHist -> do+                    let Just (jkMean, jkSE) = k `Map.lookup` jkHist+                    return $ T.intercalate " " [T.pack . showSitePattern $ k, T.pack . show $ v,+                                                T.pack . show $ jkMean, T.pack . show $ jkSE]+    return $ T.unlines (head0:head1:head2:head3:body)+  where+    sorted = sortBy (\(_, v1) (_, v2)  -> compare v2 v1) $ Map.toList (raCounts hist)++-- |Read a histogram from a FilePath+readHistogram :: FilePath -> Script RareAlleleHistogram+readHistogram path = do+    h <- scriptIO $ openFile path ReadMode+    hist <- readHistogramFromHandle h+    scriptIO $ hClose h+    return hist++-- |Read a histogram from a File Handle.+readHistogramFromHandle :: Handle -> Script RareAlleleHistogram+readHistogramFromHandle handle = do+    res <- evalStateT (parse parseHistogram) . PT.fromHandle $ handle+    case res of+        Nothing -> throwE "histogram file exhausted too early"+        Just (Left err) -> throwE $ format ("Histogram parsing error: "%w) err+        Just (Right hist) -> return hist++parseHistogram :: A.Parser RareAlleleHistogram+parseHistogram = do+    names <- parseNames+    nVec <- parseNVec+    maxM <- parseMaxM+    totalNrSites <- parseTotalNrSites+    body <- parseBody+    let countHist = Map.fromList $ [(k, c) | (k, c, _) <- body]+        jkHist = case head body of+            (_, _, Just _) -> Just . Map.fromList $ [(k, (jkMean, jkSE)) |+                                                     (k, _, Just (jkMean, jkSE)) <- body]+            _ -> Nothing+    return $ RareAlleleHistogram names nVec 1 maxM [][] totalNrSites countHist jkHist+  where+    parseNames = A.string "NAMES=" *> name `A.sepBy1` A.char ',' <* A.endOfLine+    name = A.takeWhile1 (\c -> isAlphaNum c || c == '_' || c == '-')+    parseNVec = A.string "N=" *> A.decimal `A.sepBy1` A.char ',' <* A.endOfLine+    parseMaxM = A.string "MAX_M=" *> A.decimal <* A.endOfLine+    parseTotalNrSites = A.string "TOTAL_SITES=" *> A.decimal <* A.endOfLine++parseBody :: A.Parser [(SitePattern, Int64, Maybe (Double, Double))]+parseBody = A.many1 patternLine+  where+    patternLine = (,,) <$> parsePattern <* A.space <*> parseLargeInt <*> optional parseJackknife <*+        A.endOfLine+    parsePattern = A.decimal `A.sepBy1` A.char ','+    parseLargeInt = read <$> A.many1 A.digit+    parseJackknife = (,) <$> (A.space *> A.double) <* A.space <*> A.double
+ src/SequenceFormats/Utils.hs view
@@ -0,0 +1,53 @@+{-# LANGUAGE OverloadedStrings #-}++-- |This module contains helper functions for file parsing.++module SequenceFormats.Utils (liftParsingErrors,+                              consumeProducer,+                              FormatException(..),+                              Chrom(..)) where++import Control.Exception (Exception)+import Control.Monad.Catch (MonadThrow, throwM)+import Control.Monad.Trans.Class (lift)+import qualified Data.Text as T+import qualified Data.Attoparsec.Text as A+import Pipes (Producer, next)+import Pipes.Attoparsec (ParsingError(..), parsed)++-- |A wrapper datatype for Chromosome names.+newtype Chrom = Chrom {unChrom :: T.Text} deriving (Eq)++-- |Show instance for Chrom+instance Show Chrom where+    show (Chrom c) = show c++-- |Ord instance for Chrom+instance Ord Chrom where+    compare (Chrom c1) (Chrom c2) = +        let c1' = if T.take 3 c1 == "chr" then T.drop 3 c1 else c1+            c2' = if T.take 3 c2 == "chr" then T.drop 3 c2 else c2+            cn1 = read . T.unpack $ c1' :: Int+            cn2 = read . T.unpack $ c2' :: Int+        in  cn1 `compare` cn2++-- |An exception type for parsing BioInformatic file formats.+data FormatException = FormatException T.Text+    deriving Show++instance Exception FormatException++-- |A function to help with reporting parsing errors to stderr. Returns a clean Producer over the +-- parsed datatype.+liftParsingErrors :: (MonadThrow m) =>+    Either (ParsingError, Producer T.Text m r) () -> Producer a m ()+liftParsingErrors res = case res of+    Left (ParsingError cont msg, restProd) -> do+        Right (chunk, _) <- lift $ next restProd+        let msg' = msg ++ " Error occurred while trying to parse this chunk: " ++ show chunk+        throwM (ParsingError cont msg')+    Right () -> return ()++-- |A helper function to parse a text producer, properly reporting all errors to stderr.+consumeProducer :: (MonadThrow m) => A.Parser a -> Producer T.Text m () -> Producer a m ()+consumeProducer parser prod = parsed parser prod >>= liftParsingErrors
+ src/SequenceFormats/VCF.hs view
@@ -0,0 +1,157 @@+{-# LANGUAGE OverloadedStrings #-}++{-| A module to help with parsing VCF files. The VCF format is defined here:+<https://en.wikipedia.org/wiki/Variant_Call_Format>+-}++module SequenceFormats.VCF (VCFheader(..),+                     VCFentry(..),+                     readVCFfromStdIn,+                     readVCFfromFile,+                     getGenotypes,+                     getDosages,+                     isTransversionSnp,+                     vcfToFreqSumEntry,+                     isBiallelicSnp) where++import SequenceFormats.Utils (consumeProducer, Chrom(..))+import SequenceFormats.FreqSum (FreqSumEntry(..))++import Control.Applicative ((<|>), empty)+import Control.Error (headErr, assertErr)+import Control.Monad (void)+import Control.Monad.Catch (MonadThrow, throwM)+import Control.Monad.Trans.State.Strict (runStateT)+import Control.Monad.IO.Class (MonadIO)+import qualified Data.Attoparsec.Text as A+import Data.Char (isSpace)+import qualified Data.Text as T+import Pipes (Producer)+import Pipes.Attoparsec (parse, ParsingError(..))+import Pipes.Safe (MonadSafe)+import qualified Pipes.Text.IO as PT++-- |A datatype to represent the VCF Header. Most comments are simply parsed as entire lines, but the very last comment line, containing the sample names, is separated out+data VCFheader = VCFheader {+    vcfHeaderComments :: [T.Text], -- ^A list of containing all comments starting with a single '#'+    vcfSampleNames :: [T.Text] -- ^The list of sample names parsed from the last comment line +                             -- starting with '##'+} deriving (Show)++-- |A Datatype representing a single VCF entry.+data VCFentry = VCFentry {+    vcfChrom :: Chrom, -- ^The chromosome+    vcfPos :: Int, -- ^The position+    vcfId :: Maybe T.Text, -- ^The SNP ID if non-missing+    vcfRef :: T.Text, -- ^ The reference allele (supports also multi-character alleles for Indels)+    vcfAlt :: [T.Text], -- ^The alternative alleles, each one possible of multiple characters +    vcfQual :: Double, -- ^The quality value+    vcfFilter :: Maybe T.Text, -- ^The Filter value, if non-missing.+    vcfInfo :: [T.Text], -- ^A list of Info fields+    vcfFormatString :: [T.Text], -- ^A list of format tags+    vcfGenotypeInfo :: [[T.Text]] -- ^A list of format fields for each sapmle.+} deriving (Show)++readVCFfromProd :: (MonadThrow m) =>+    Producer T.Text m () -> m (VCFheader, Producer VCFentry m ())+readVCFfromProd prod = do+    (res, rest) <- runStateT (parse vcfHeaderParser) prod+    header <- case res of+        Nothing -> throwM $ ParsingError [] "freqSum file exhausted"+        Just (Left e) -> throwM e+        Just (Right h) -> return h+    return (header, consumeProducer vcfEntryParser rest)++-- |Reading a VCF from StdIn. Returns a VCFHeader and a Producer over VCFentries.+readVCFfromStdIn :: (MonadIO m, MonadThrow m) => m (VCFheader, Producer VCFentry m ())+readVCFfromStdIn = readVCFfromProd PT.stdin++-- |Reading a VCF from a file. Returns a VCFHeader and a Producer over VCFentries.+readVCFfromFile :: (MonadSafe m) => FilePath -> m (VCFheader, Producer VCFentry m ())+readVCFfromFile = readVCFfromProd . PT.readFile++vcfHeaderParser :: A.Parser VCFheader+vcfHeaderParser = VCFheader <$> A.many1' doubleCommentLine <*> singleCommentLine+  where+    doubleCommentLine = do+        c1 <- A.string "##"+        s_ <- A.takeWhile1 (not . A.isEndOfLine)+        A.endOfLine+        return $ T.append c1 s_+    singleCommentLine = do+        void $ A.char '#'+        s_ <- A.takeWhile1 (not . A.isEndOfLine)+        A.endOfLine+        let fields = T.splitOn "\t" s_+        return . drop 9 $ fields++vcfEntryParser :: A.Parser VCFentry+vcfEntryParser = VCFentry <$> (Chrom <$> word) <* sp <*> A.decimal <* sp <*> parseId <* sp <*>+    word <* sp <*> parseAlternativeAlleles <* sp <*> A.double <* sp <*> parseFilter <* sp <*> +    parseInfoFields <* sp <*> parseFormatStrings <* sp <*> parseGenotypeInfos <* A.endOfLine+  where+    word = A.takeTill A.isHorizontalSpace+    sp = A.satisfy A.isHorizontalSpace+    parseId = parseDot <|> (Just <$> word)+    parseDot = A.char '.' *> empty+    parseAlternativeAlleles = parseDot <|> (parseAllele `A.sepBy1` A.char ',')+    parseAllele = A.takeTill (\c -> c == ',' || A.isHorizontalSpace c)+    parseFilter = parseDot <|> (Just <$> word)+    parseInfoFields = parseDot <|> (parseInfoField `A.sepBy1` A.char ';')+    parseInfoField = A.takeTill (\c -> c == ';' || A.isHorizontalSpace c)+    parseFormatStrings = A.takeTill (\c -> c == ':' || A.isHorizontalSpace c) `A.sepBy1` A.char ':'+    parseGenotypeInfos = parseGenotype `A.sepBy1` (A.satisfy A.isHorizontalSpace)+    parseGenotype = parseGenoField `A.sepBy1` (A.char ':')+    parseGenoField = A.takeTill (\c -> c == ':' || isSpace c) ++-- |returns True if the SNP is biallelic.+isBiallelicSnp :: T.Text -> [T.Text] -> Bool+isBiallelicSnp ref alt = validRef && validAlt+  where+    validRef = (ref `elem` ["A", "C", "G", "T"])+    validAlt = case alt of+        [alt'] -> alt' `elem` ["A", "C", "G", "T"]+        _ -> False++-- |returns True if the SNp is a biallelic Transversion SNP (i.e. one of G/T, G/C, A/T, A/C)+isTransversionSnp :: T.Text -> [T.Text] -> Bool+isTransversionSnp ref alt =+    case alt of+        [alt'] -> isBiallelicSnp ref alt && (not $ isTransition ref alt')+        _ -> False+  where+    isTransition r a = ((r == "A") && (a == "G")) || ((r == "G") && (a == "A")) ||+                       ((r == "C") && (a == "T")) || ((r == "T") && (a == "C"))++-- |Extracts the genotype fields (for each sapmle) from a VCF entry+getGenotypes :: VCFentry -> Either String [T.Text]+getGenotypes vcfEntry = do+    gtIndex <- fmap fst . headErr "GT format field not found" . filter ((=="GT") . snd) .+               zip [0..] . vcfFormatString $ vcfEntry+    return $ map (!!gtIndex) (vcfGenotypeInfo vcfEntry)++-- |Extracts the dosages (the sum of non-reference alleles) per sample (returns a Left Error if it fails.)+getDosages :: VCFentry -> Either String [Maybe Int]+getDosages vcfEntry = do+    genotypes <- getGenotypes vcfEntry+    let dosages = do+            gen <- genotypes+            if '.' `elem` (T.unpack gen) then+                return Nothing+            else+                return . Just $ T.count "1" gen+    return dosages++-- |Converts a VCFentry to the simpler FreqSum format (returns a Left Error if it fails.)+vcfToFreqSumEntry :: VCFentry -> Either String FreqSumEntry+vcfToFreqSumEntry vcfEntry = do+    dosages <- getDosages vcfEntry+    assertErr "multi-site reference allele" $ T.length (vcfRef vcfEntry) == 1+    assertErr "need exactly one alternative allele" $ length (vcfAlt vcfEntry) == 1+    assertErr "multi-site alternative allele" $ T.length (head . vcfAlt $ vcfEntry) == 1+    let ref = T.head (vcfRef vcfEntry)+    let alt = T.head . head . vcfAlt $ vcfEntry+    assertErr "Invalid Reference Allele" $ ref `elem` ['A', 'C', 'T', 'G', 'N']+    assertErr "Invalid Alternative Allele" $ alt `elem` ['A', 'C', 'T', 'G', '.']+    return $ FreqSumEntry (vcfChrom vcfEntry) (vcfPos vcfEntry) ref alt dosages+